The effect of Dimethyl sulfoxide on facilitating the absorption of hepatitis B virus in HepG 2 cells
10.3969/j.issn.1673-4130.2015.23.034
- VernacularTitle:二甲基亚砜促进乙型肝炎病毒对 HepG2细胞的吸附作用
- Author:
Linglong REN
;
Yongjian GUO
;
Yulan LUO
- Publication Type:Journal Article
- Keywords:
hepatitis B virus;
dimethyl sulfoxide;
HepG2 cells;
adsorption
- From:
International Journal of Laboratory Medicine
2015;(23):3444-3446
- CountryChina
- Language:Chinese
-
Abstract:
Objective To preliminarily explore the early process of hepatitis B virus (HBV) infection in HepG2 cells induced by dimethyl sulfoxide(DMSO) ,and provide cytological bases for mechanism study of HBV infection in vitro .Methods HepG cells were divided into the DMSO inducing group and control group ,and were cultured 4 days by DMEM containing 1 .5% DMSO and normal DMEM respectively ;changes of cellular morphology were observed .In addition ,selected ECV304 cells as the negative con‐trol group and treated with DMSO .Cells in the three groups were incubated 2 hours with HBV positive serum after culturing 24 hours ,then trypsin digestive solution ,HepG2 cells and ECV304 cells were collected and polymerase chain reaction (PCR) was used for the determination of HBV DNA .Simultaneously ,the blank control group was set ,and the position of HBsAg on HepG2 cells were detected by using indirect immunofluorescence .Results HepG2 cell volume in the DMSO inducing group was obviously in‐creased .HBV DNA was found in HepG2 cells both in DMSO inducing group and control group ,and DMSO inducing group ex‐pressed much stronger .The results of IIF shown that green fluorescent signals of cell membrane and cytoplasm of HepG 2 cells in the DMSO inducing group were increased obviously ,while the results of HBV DNA and IIF both were negative in the negative con‐trol group .Conclusion DMSO could facilitate adsorption of HBsAg in some extent ,which are benefit for completing the process of early infection .
