Typing of Vibrio parahaemolyticus strains from aquatic products in East-China coastal areas based on multilocus sequence typing and AA-multilocus sequence typing
10.7644/j.issn.1674-9960.2015.11.001
- VernacularTitle:我国东部沿海地区水产品中副溶血弧菌分离株的基于碱基序列和肽链序列的多位点序列分型研究
- Author:
Jialiang XU
;
Xiaoli DU
;
Biao KAN
;
Xin LU
- Publication Type:Journal Article
- Keywords:
Vibrio parahaemolyticus;
multilocus sequence typing;
peptide based multilocus sequence typing;
clonal complex
- From:
Military Medical Sciences
2015;(11):811-815
- CountryChina
- Language:Chinese
-
Abstract:
Objective To learn more about virulence genes and clonal complex group structure of Vibrio parahaemolyticus (VPH)strains separated from aquatic products in East China coastal areas between 2007 and 2012.Methods Seventy-nine strains separated from aquatic products in eastern coastal areas(Guandong,Fujian,Shanghai,Shandong,Jiangsu and Beijing)of China between 2007 and 2012 were identified as VPH by real-time-PCR with gene tlh.Gene tdh,trh and orf8 were also detected.Subgroup analysis and complex analysis were conducted of the VPH strains to build the minimum spanning tree respectively based on ST and pST types.Results In 79 VPH strains,gene tlh was positive while 8.86%(7 /79)of the isolates of gene tdh were positive.The carrying rate of gene orf8 was 8.86%.These 79 strains were of 69 ST types,involving 3 clonal complex and 62 singleton.By amino acid(AA)-multilocus sequence typing(MLST),79 strains covered the 23 pST types,2 clonal complexes and 1 singleton.The 363 single nucleotide polymorphisms(SNPs)were divided into 68 patterns.Conclusion VPH strains from aquatic products in eastern coastal areas of China are characterized by high polymorphisms and a low carrying rate of virulence genes.ST3 type is dominating when MLST typing is used while pST1 type is the majority by AA-MLST typing.
