Comparative study on fluorescent quantitation polymerase chain reaction and gene-chips typing method in genotyping HPV
10.3969/j.issn.1673-4130.2015.23.009
- VernacularTitle:荧光定量聚合酶链式反应和基因芯片分型法行HPV分型检测的比较研究
- Author:
Xiurong LONG
;
Jianyun LAN
;
Jianxiang GENG
;
Xuemei FAN
;
Lin XIA
;
Hongjing WANG
;
Jing MEI
;
Xue ZHAO
- Publication Type:Journal Article
- Keywords:
human papillomavirus;
fluorescent quantitation polymerase chain reaction;
gene-chips typing method
- From:
International Journal of Laboratory Medicine
2015;(23):3385-3387
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the sensitivity of fluorescent quantitation polymerase chain reaction (fluorescent quantitation method) and gene‐chips typing method(gene‐chips method) in the detection of human papillomavirus(HPV) ,and to analyse differ‐ences and clinical significance .Methods A total of 246 women were selected as subjects ,among them ,111 cases of cervical exfolia‐ted cells and 135 cases of cervical tissues were collected and detected .15 kinds of high‐risk HPV genetypes were detected in all sub‐jects by using fluorescent quantitation method and gene‐chips method respectively ,and the detection results were compared . Results The sensitivity of the fluorescent quantitation method in detecting HPV was 55 .28% and that of the gene‐chips method was 55 .69% ,there was no statistically significant difference in sensitivity between the two methods (P>0 .05) .The two methods had relative high conformance(κ=0 .745) .The positive rate of HPV infection was increased with the progression of cervical dis‐ease .Conclusion The fluorescent quantitation method and the gene‐chips method have a relative high conformance ,and both with high sensitivity in detecting HPV .The severity degree of cervical cytological and histological changes may be positively correlated with HPV infection .
