Inducement Effect of Interferon Alpha on Apoptosis of Rat Vascular Smooth Muscle Cells via P204 and RAS Signal Pathway
10.3870/j.issn.1004-0781.2015.12.002
- VernacularTitle:干扰素α通过P204和RAS信号途径诱导大鼠血管平滑肌细胞凋亡
- Author:
Xiangshu LONG
;
Qiang WU
;
Taihe LIU
;
Fang SONG
;
Jing HUANG
;
Maobo TIAN
;
Yan XIAO
- Publication Type:Journal Article
- Keywords:
Interferon;
Vascular smooth muscle cells;
Interferon induction protein 204;
RAS signal pathway;
Apoptosis
- From:
Herald of Medicine
2015;34(12):1555-1558
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of interferon alpha ( IFN-α) on apoptosis of vascular smooth muscle cells ( VSMCs) in rats and the related mechanism. Methods The cells were divided into three group:group A, group B and group C.Group A was transfected with nonspecific siRNA, group B was intervened with IFN-α and transfected with nonspecific siRNA, and group C was intervened with IFN-α and transfected with IFI204 siRNA. All the cells were cultured for 48 h. The expression of P204 mRNA was determined by semiquantitative reverse transcription polymerase chain reaction (RT-PCR).P204, RAS protein levels, and phosphorylation levels of RAF and ERK were analyzed by Western blotting. The cell apoptosis was analyzed by flow cytometry with Annexin-V FITC/PI method. Results As compared with group A, the expression of P204 mRNA and protein in group B was up-regulated (P<0.05), the cell apoptosis was increased (P<0.05), in the process of the above, the expression of RAS protein was decreased ( P<0.05) and the phosphorylation levels of RAF and ERK were dropped (P<0.05).In group C, the expression levels of P204 mRNA and protein were down-regulated (P<0.05), and cell apoptosis was decreased ( P<0.05) , the expression of RAS protein and the phosphorylation levels of RAF and ERK were increased ( P<0.05) . Conclusion P204 and RAS signal pathway participates in IFN-α regulation of apoptosis of VSMCs in rats.
