Effects of TNF-α and TGF-β1 on Gli2 expression in primary leukemia cells
10.3969/j.issn.1006-5725.2015.22.015
- VernacularTitle:肿瘤坏死因子-α联合转化生长因子-β1作用于原代白血病细胞对Gli2表达的影响
- Author:
Zhe LI
;
Yuanyuan SUN
;
Jing PAN
- Publication Type:Journal Article
- Keywords:
Primary leukemia cells;
TGF-β1;
TNF-α;
Gli2
- From:
The Journal of Practical Medicine
2015;31(22):3678-3681
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether TNF-α can promote, but TGF-β can reduce Gli2 expression in primary leukemia cells. Methods (1)Western blot was used to detect Gli2 protein expression in leukemic cells. (2)Primary leukemia cells were treated with TGF-β1 or TNF-α,and expressions of Gli2 and TGF-βR were detected. (3)Primary leukemia cells were treated with TGF-β1,TNF-α or/and SIS3, and Gli2 protein expression was detected. Results (1)The primary leukemia cells could express Gli2 protein. (2)Significant reduction of Gli2 mRNA was observed in 1 ~ 10 ng/mL TGF-β1-treated primary leukemia cells for 12 ~ 24 h, No significant differences of Gli2 mRNA was found between the control group and TNF-α group. (3)Protein expresson of TGF-βRⅠand TGF-βRⅡ in 5 ng/mL TNF-α-treated primary leukemia cells for 24 h was higher than that of the control group. (4)Gli2 portein expression was lower in TGF-β1 with or without TNF-α-treated primary leukemia cells for 24 h than that in the control group, and Gli2 portein expression in the TGF-β1 plus TNF-α group was lower than that in TGF-β1 group. Conclusion Combinations of TGF-β and TNF-α can efficiently inhibit Gli2 expression than TGF-β alone in primary leukemia cells.
