BRCA1 microsatellite instability in endometrial carcinoma and its relationship with clinical pathology
10.3969/j.issn.1006-5725.2015.22.027
- VernacularTitle:子宫内膜癌中人类乳腺癌易感基因1微卫星不稳定性及其与临床病理的关系
- Author:
Dan SUN
;
Yujuan FAN
;
Hong XU
;
Jiangtao FAN
- Publication Type:Journal Article
- Keywords:
Endometrial cancer;
Breast cancer susceptibility gene 1;
Microsatellite instability;
Pathology;
PCR-SSCP
- From:
The Journal of Practical Medicine
2015;31(22):3717-3719
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the occurrence of BRCA1 MSI in endometrial cancer and its relationship with clinic pathologic features; to explore the correlation between MSI and protein expression in BRCA1 gene. Methods Application of PCR-SSCP and DNA sequence analysis method was used to study D17S579 and D17S1349 in 49 sporadic endometrial cancer tissues, 20 cases with endometrial atypical hyperplasia and 28 cases with normal endometrial tissues. Results In the total samples of D17S579 and D17S1349, the three groups were significantly different: 34.69%(17/49) in the endometrial cancer group, 10%(2/20) in the endometrial atypical hyperplasia group and 7.14%(2/28) in the normal endometrium group (χ2= 11.208, P = 0.004). BRCA1 MSI positive rate related to the pathology grade and clinical stage, but no relationship was found in muscular infiltration depth, lymph node metastasis and histopathology types. In the endometrial cancer group, BRCA1 MSI positive rate and BRCA1 protein expression were in moderate correlated negatively (r = -0.779, P = 0.000). Conclusion BRCA1 MSI might play a role in the development of endometrial cancer, and low expression of BRCA1 protein. BRCA1 MSI might be associated with pathology grade and clinical stages in EC.
