HPLC Fingerprint Analysis of Disporum Cantoniense
10.3870/j.issn.1004-0781.2015.12.019
- VernacularTitle:百尾参药材高效液相色谱指纹图谱分析
- Author:
Xiuhai GAN
;
Zhiyuan LIANG
;
Xin ZHOU
;
Chao ZHAO
;
Gang WEI
- Publication Type:Journal Article
- Keywords:
Disporum cantoniense;
Chromatography;
high performance liquid;
Fingerprint;
Similarity;
Hierarchical cluster analysis
- From:
Herald of Medicine
2015;34(12):1623-1627
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the HPLC fingerprint of Disporum cantoniense. Methods HPLC analysis was performed on Agilent Zorbax SB-C18 chromatographic column ( 250 mm × 4. 6 mm, 5 μm) with the mobile phase consisting of methanol-0.05% phosphoric acid in gradient mode.The flow rate was 1.0 mL·min-1, the detection wavelength was 256 nm and the column temperature was 30 ℃. Results The HPLC fingerprint of 15 batches of Disporum cantoniense was established. Thirteen common peaks in the fingerprint were demarcated, four of which were identified by reference substances. Chemical pattern recognition of fingerprint was performed by hierarchical cluster analysis and principal component analysis. Conclusion The method is simple, accurate and has a good repeatability, and can be used for quality control of Disporum cantoniense.
