A new method of mouse tracheal instillation with endoscope of cold light and gel loading tips
10.3969/j.issn.1001-1978.2016.04.028
- VernacularTitle:应用冷光源内窥镜和点样吸头完成的新型小鼠气管滴注法
- Author:
Biao YANG
;
Xinming LI
;
Siyuan YAN
;
Dongmei CHEN
;
Jiayu NIU
;
Chunling XIAO
- Publication Type:Journal Article
- Keywords:
new method of endotracheal instillation;
endoscope of cold light;
gel loading tips;
mice;
experimental techniques;
endotracheal intubation
- From:
Chinese Pharmacological Bulletin
2016;32(4):585-587
- CountryChina
- Language:Chinese
-
Abstract:
Aim To develop a new method for easy endotrache-al intubation and instillation in mice. Methods Relying on the endoscope of cold light and gel loading tips, a new method was set up for intubated operations. Under high magnification, oper-ations of endotracheal intubation and instillation were completed in mice. Twenty male CB17-SCID mice ( between 24 and 28 g) were repeatedly intubated and instilled with 80 μL suspensions of air polluted fine particulate matters in a week. Results The twenty mice were sacrificed after 24h of the last instillation. The dispersions of the air polluted fine particulate matters were found at the bottoms of tracheas and in both the left and right lungs in all mice. Conclusion Using the endoscope of cold light and gel loading tips, a new method for tracheal instillation is set up in mice, which is rapid, simple and has good repeatability. It could be widely applied for the endotracheal intubation and in-stillation in mice.
