The influence of bFGF gene transfected BMSCs on inflammatory cytokines expression of COPD rat
10.3969/j.issn.1006-5725.2016.03.005
- VernacularTitle:bFGF基因转染BMSCs对COPD大鼠肺部炎症因子表达的影响
- Author:
Peng WANG
;
Qiang NIE
;
Lin MA
;
Shanshan PENG
;
Xin GAN
- Publication Type:Journal Article
- Keywords:
Basic fibroblast growth factor;
Bone marrow-derived mesenchymal stem cells;
Chronic Obstructive Pulmonary Diseases;
Tumor necrosis factor-α;
Interleukin-1β
- From:
The Journal of Practical Medicine
2016;32(3):355-358
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of bFGF gene transfected bone marrow-derived mesenchymal stem cells (BMSCs) on the inflammatory cytokines of COPD rat. Methods The BMSCs were separated from SD rat and cultured and then bFGF gene was imported to BMSCs by liposome transfection method. The samples were prepared into six groups: normal control group, COPD group (A), BMSCs group (B), pcDNA3.1-BMSCs group (C), bFGF-pcDNA3.1-BMSCs group (D), and bFGF group (E). The expressions of TNF-α and IL-1β by QRT-PCR were detected. Results Compared with COPD group, TNF-α and IL-1β genes from groups B to D dropped significantly (P < 0.05). The changes of TNF-α and IL-1β genes among groups B to D showed no significant difference (P > 0.05). Conclusion BFGF transfected BMSCs, sample BMSCs and pcDNA3.1 transfected BMSCs can inhibit the expression of inflammatory cytokines of TNF-α and IL-1β, but there is no obvious advantage in comparison to bFGF transfected BMSCs and sample BMSCs in respect of inhibiting the expression of inflammatory cytokines of TNF-α and IL-1β.
