Effect of bone marrow stromal cell and its supernatant on expression of synaptophysin in hippocampal dentate gyrus from rats with hypoxic-ischemic brain damage and its mechanism
10.3969/j.issn.1006-5725.2016.03.006
- VernacularTitle:骨髓基质细胞及其上清液对缺氧缺血脑损伤大鼠海马齿状回突触素表达的影响及机制
- Author:
Xiaoming ZHONG
;
Hong YU
;
Yan HE
;
Kaiyuan LUO
;
Hongqun LIAO
;
Changpu WANG
- Publication Type:Journal Article
- Keywords:
Bone marrow transplantation;
Bone marrow cells;
Hypoxia-ischemia,brain;
Synapses;
Cytokines
- From:
The Journal of Practical Medicine
2016;32(3):359-361
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the expression of synaptophysin (SY) in hippocampal dentate gyrus of rats with hypoxic-ischemic brain damage(HIBD) after the injection of bone marrow stromal cells(BMSCs) and its supernatant into the lateral ventricle and to explore the mechanism of potential effects. Methods Eighty 7-day-old sprague-dawley(SD) rats were randomly divided into sham group, control group, cell group and supernatant group, with 20 rats in each group. The HIBD model was established via the ligation of left carotid arteries followed by 2-hour hypoxia. One week later, control group was injected with 0.01 mol/L PBS via the left ventricle. Meanwhile, cell and supernatant group were injected with BMSCs and supernatant harvested from BMSC culture, respectively, via the same route. For spam group, the left carotid arteries were separated but not ligated, and no hypoxia treatment was imposed on this group. They also received 0.01mol/L PBS injection one week post surgery. After 8 weeks, the expression of SY in the dentate gyrus of rats was measured. Results Compared with sham group, model group showed significantly lower IOD (integral optical density) of SY positive material in the dentate gyrus(P < 0.05). Compared with model group, supernatant group and cell group exhibited significantly increased IOD values (P < 0.05), but there was no significant difference between cell group and supernatant group (P > 0.05). Conclusions BMSCs and supernatant injected via the lateral cerebral ventricle can increase SY expression in the hippocampal dentate gyrus of HIBD rats and promote synapse formation. The mechanism of this effect may be related to cytokine secretion by BMSCs which promotes synapse formation.
