Effect of homocysteine on the intestinal permeability by regulating MEK-ERK-MLCK signal transduction in experimental colitis rats
10.3969/j.issn.1001-1978.2016.04.012
- VernacularTitle:同型半胱氨酸调控MEK-ERK-MLCK通路影响结肠炎大鼠肠黏膜通透性的实验研究
- Author:
Shaozhen DING
;
Hao DING
;
Qiao MEI
;
Xiaochang LIU
;
Jing HU
;
Yongmei HU
;
Jianming XU
- Publication Type:Journal Article
- Keywords:
homocysteine;
inflammatory bowel dis-ease;
permeability;
tight junction;
myosin light chain kinase;
extracellular regulated protein kinase
- From:
Chinese Pharmacological Bulletin
2016;32(4):498-502
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate whether Hcy influenced the intestinal mucosal permeability by regulating MEK-ERK-MLCK pathway. Methods SD rats were divided into 4 groups:normal group, normal+Hcy group, TN-BS/ethanol group, TNBS/ethanol+Hcy group. Experi-mental colitis model with hyperhomocystinemia was es-tablished in rats with intracolonic administration of TN-BS and subcutaneous injection of Hcy. The colonic mucosal tissue was collected for histopathological exam-ination and activity of myeloperoxidase ( MPO ) . The protein expression of MLCK, p-MLCK, MEK, ERK and p-ERK in intestinal mucosal tissues was examined by Western blot method. The mRNA expression of ML-CK was examined by RT-qPCR method. Result Com-pared with the normal group and TNBS group, the DAI and HI scores and the MPO activity were increased in TNBS/ethanol+Hcy group ( P <0. 01 ) . Western blot and RT-qPCR showed that expression of MLCK, p-ML-CK, MEK, ERK and p-ERK increased in small intes-tine in TNBS/ethanol+Hcy group. Conclusion Hcy can increase intestinal permeability in TNBS-induced colitis rats by regulating the expression of MEK-ERK-MLCK signal pathway.
