Effects of diterpenoid pekinenal of Euphorbia pekinensis Rupr. on proliferation,cell cycle and apoptosis of hepatoma cells
10.3969/j.issn.1001-1978.2016.04.016
- VernacularTitle:京大戟中二萜类化合物pekinenal对肝癌细胞增殖、周期和凋亡的影响
- Author:
Feiyan CHEN
;
Weiwei TAO
;
Kouyu CHEN
;
Jiaqing ZHANG
;
Yuqing WANG
;
Gang CHEN
;
Jinao DUAN
- Publication Type:Journal Article
- Keywords:
Euphorbia pekinensis Rupr;
pekinenal;
SMMC-7721;
cell proliferation;
cell cycle;
cell apopto-sis
- From:
Chinese Pharmacological Bulletin
2016;32(4):519-524
- CountryChina
- Language:Chinese
-
Abstract:
Aims To study the effects of diterpenoid pekinenal of Euphorbia pekinensis Rupr. on cell prolif-eration, cell cycle phase and apoptosis of hepatoma SMMC-7721 cells and to probe into its anti-cancer mechanisms. Methods MTT assay was used to inves-tigate the effect of pekinenal on proliferation of SMMC-7721 cells; TUNEL method, Annexin V/PI staining and electron microscopy were employed to observe the cell apoptosis; Flow cytometry was applied to analyze the distribution of cell cycle. Results Proliferation of SMMC-7721 cells was markedly inhibited by pekinenal in a dose-dependent manner; Annexin V/PI staining showed that with the increase of pekinenal concentra-tion, apoptotic rate of SMMC-7721 cells increased sig-nificantly, compared with control group, the difference has significant statistical significance ( P < 0. 01 ) . TUNEL method test results showed that different con-centrations of pekinenal in SMMC-7721 cells could in-duce liver cancer cell apoptotic and apoptotic index ( AI) increased significantly ( P <0. 01 ) with the in-crease of drug concentration. Compared with control group, electron microscope found that after the treat-ment, hepatocyte mitochondrial swelling, endoplasmic reticulum expansion, cytoplasm inclusion body forma-tion, part of the nucleus apoptosis, and the more obvi-ous apoptosis appeared with the increase of drug con-centration. Flow cytometry analysis showed that differ-ent concentrations of pekinenal could all make the cell block in S phase. Conclusion Pekinenal has an obvi-ously inhibitory effect on the human liver cancer cells, and there is significant concentration dependence; Pe-kinenal probably inhibits cancer cell DNA synthesis for the human liver cell cycle arrest in S phase and inhibits the proliferation . It plays a role in liver cancer inhibi-tion by inducing liver cancer cells apoptosis, etc.
