Effects of lipopolysaccharide on lung inj ury and expressions of AQP1 and AQP5 in lung tissue of rats
10.13481/j.1671-587x.20160212
- VernacularTitle:脂多糖对大鼠肺损伤及肺组织中AQP 1和AQP5表达的影响
- Author:
Guobing CHEN
;
Jinzhun WU
;
Zhulan LIAN
;
Zhuqin ZHAN
;
Haitao BAI
- Publication Type:Journal Article
- Keywords:
lipopolysaccharide;
lung inj ury;
aquaporins 1;
aquaporins 5
- From:
Journal of Jilin University(Medicine Edition)
2016;42(2):250-254
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of lipopolysaccharide (LPS)on the acute lung injury (ALI)and expressions of aquaporin 1 (AQP1)and aquaporin 5 (AQP5)in lung tissue of the rats. Methods:Forty-eight SPF grade male Wistar rats were randomly divided into control group and LPS group (n=24).The rats in LPS group were intravenously injected with LPS to induce ALI models,and the rats in control group were injected with saline. The rats were sacrificed at 2,6,12 and 24 h,and the samples were collected after the successful modeling.The pathological changes of lung tissue were observed with HE staining;the lung wet/dry weight (W/D)ratio and lung permeability index were detected;ELISA was used to detect the levels of TNF-αand MIP-1α.The expression levels of AQP1 and AQP5 protein and mRNA were measured by Western blotting,immunohistochemistry and Real-Time PCR methods. Results:Compared with control group, the TNF-α and MIP-1α levels in LPS group were significantly elevated at 2,6 and 12 h (P<0.05),and at 24 h they were gradually reduced to the normal level. The HE staining results showed the alveolar and interstitial edema at 2 h after LPS injection,obviously in 12 h. The lung W/D ratios and pulmonary permeability indexes at different time points in LPS group were significantly higher than those in control group (P<0.05),and they reached the peak at 12 h.The expression levels of AQP1 and AQP5 mRNA and protein in lung tissue of the rats at different time points in LPS group were significantly lower than those in control group (P<0.01 ). Conclusion:LPS can induce ALI in the rats and down-regulate the expressions of AQP1 and AQP5;LPS is involved in the formation of pulmonary edema.
