The analysis of gene expression profile and related signal pathways in focal cortical dysplasia
10.3969/j.issn.1006-5725.2016.03.003
- VernacularTitle:局灶性皮质发育不良基因表达及相关信号通路分析
- Author:
Lidong HUA
;
Wenbin LI
;
Quwen GAO
;
Lisen SUI
;
Cuixia FAN
;
Xiaoming OUYANG
;
Weiping LIAO
;
Bingmei LI
;
Yiwu SHI
- Publication Type:Journal Article
- Keywords:
Focal cortical dysplasia;
Gene expression;
Lysophosphatidic acid;
Signal pathway;
Myelin
- From:
The Journal of Practical Medicine
2016;32(3):347-351
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the potential pathogenesis of Focal cortical dysplasia (FCD), we performed cDNA microarray analysis to obtain gene expression profile of FCD. Methods Three FCD samples and three normal controls were enrolled. Total RNA of the brain tissues were extracted. The difference gene expressions between FCD group and control group was detected using Affymetrix gene chip. The up and down-regulated genes were confirmed by Real-time PCR. Further, the related signal pathways involved in the pathogenic mechanisms of FCD were predicted by bioinformatics. Result In FCD, two up-regulated genes C21orF2 and AU152162 and 5 down-regulated genes ENPP2, ANLN, IP6K3, UGT8, and AZGP were found. Compared the FCD samples with the normal controls , there were significantly different in all down-regulated genes (P < 0.05), while the up-regulated genes were not (P > 0.05). Using bioinformatics analysis, the ENPP2 , UGT8 , and AZGP1 protein which located in the cell membrane or secreted into the extracellular matrix may be involved in the formation of the myelin sheath and the development of the nervous system by the lipid metabolism and LPA signaling pathway. Conclusion ENPP2, UGT8 and AZGP1 may be involved in pathogenesis of FCD through the process of myelin sheath formation and LPA signal pathway , which warrants further study to know their roles in the pathogenesis of FCD.
