Extract of HUANGPI inhibits secretion of TNF-α via TLR4/MyD88/TRAF6 pathway

VernacularTitle:黄皮叶提取物对TNF-α分泌的影响及其机制
Author: Juan LI ; Tianxu LIU ; Guojun JIANG ; Guihong HUANG ; Junhe HUANG ; Liqun TAO ; Zhaoming ZHU
Publication Type:Journal Article
Keywords: Extracts; Inflammatory factors; Signaling pathway; RAW264.7 cell
From: The Journal of Practical Medicine 2016;32(3):367-370
CountryChina
Language:Chinese
Abstract: Objective To investigate whetherthe extract of HUANGPI inhibitthe secretion of TNF-αvia TLR4/MyD88/TRAF6 signaling pathway. Methods ELISA assay was performed to determine TNF-α level in cell culture medium. MTT assay was used to detect the effects of the extract of HUANGPI and LPS on the viabilities of RAW 264.7 cells. Proteinexpressions of TLR4 and TRAF6 were detected by Western blotting assay. Results The extract of HUANGPI inhibited the secretion of TNF-αin a dose-dependent manner. Compared to LPS group , were TNF-αwas significantly suppressed in the cells in LPS+MyD88 inhibitor group , LPS+extract group and LPS+extract+MyD88 inhibitor group,with the corresponding reductions of TLR4 and TRAF6 protein expression at74% and21%,70% and27%,44% and8.5%, respectively. Conclusion MYD88-dependent signaling pathway might be involved in the mechanism underlying the effect of the extract of HUANGPI on suppressing LPS-induced inflammation.