Cytokines expression and significance in the peripheral blood from mice with temporal lobe epileptic in the acute phase
10.3969/j.issn.1002-0152.2016.01.003
- VernacularTitle:颞叶癫痫小鼠外周血细胞因子mRNA水平变化
- Author:
Yuan ZHOU
;
Lide JIN
;
Min ZHAO
;
Xia CAO
;
Xiying QIAN
- Publication Type:Journal Article
- Keywords:
Epilepsy;
Peripheral blood;
Cytokines
- From:
Chinese Journal of Nervous and Mental Diseases
2016;42(1):11-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression changes of IL-1β、FN-α6、IFN-γ and TNFR-m18 in peripheral blood within 2 hours in epileptic mice. Methods Semi quantitative real-time PCR was used to test the mRNA expres?sion level of IL-1β、FN-α6、IFN-γand TNFR-m18 in peripheral blood from normal and pilocarpine-induced epileptic mice at different time points (10 min, 30 min, 1 h and 2 h). Results The mRNA expression level of IL-1βincreased at 30 min(1.8±0.07), 1 h(2.9±0.98)and 2 h(1.45±0.11)after pilocarpine-induced status epilepticus comparing with that of control and SE 10 min(0.81±0.09)(P<0.05). The IFN-α6 mRNA expression level was lower at 10 min(0.59±0.05, P<0.05) than that of control. IFN-γmRNA expression level was higher at 10 min(2.85±0.11) than that of control and at oth?er time points during SE(P<0.01). TNFR-m18 mRNA expression level was higher at 1h(2.84±0.15) than that of control, and at other time points during SE(P<0.01). Conclusion The immune system of epileptic state is active, the imbalance of cytokine expression in peripheral blood may be related to the immune pathological process of acute stage of epilepsy.
