Effect of Fosinppril on Myocardial Cell Apoptosis and Apoptosis-associated Gene Expression in Chronic Heart Failure Rats
10.3969/j.issn.1000-3614.2016.03.019
- VernacularTitle:福辛普利对慢性心力衰竭大鼠心肌细胞凋亡及相关基因表达的影响
- Author:
Yaqin XIE
;
Juan ZHAO
;
Xiuhua LI
;
Xiangyu FENG
- Publication Type:Journal Article
- Keywords:
Fosinopril;
Hear failure;
Apoptosis;
Apoptosis-associated gene
- From:
Chinese Circulation Journal
2016;31(3):285-288
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of fosinppril on myocardial cell apoptosis and apoptosis-associated gene expression in chronic heart failure (CHF) rats.
Methods: CHF model was established by partially banding of abdominal aorta superior to renal artery. The experimental rats were randomly divided into 3 groups: Sham operation group and CHF group, the rats in both groups received stomach normal saline; Fosinopril group, the rats received stomach fosinopril 10 mg/kg?d.n=10 in each group and all animals were treated for 8 weeks. LVEDP, ±dp/dtmax and LVMI were examined, left ventricular myocardial cell apoptotic index (AI) was measured by TUNEL method, Bcl-2 and Bax protein levels were detected by immunohistochemistry and caspase-3 protein expression was assessed by Western blotting.
Results: Compared with Sham operation group, CHF group had increased LVEDP, LVMI, AI, elevated protein expressions of Bax and Caspase-3,P<0.01; while decreased ±dp/dtmax, reduced protein expression of Bcl-2 and the ratio of Bcl-2/Bax,P<0.01. Compared with CHF group, Fosinopril group presented decreased LVEDP, LVMI, AI, reduced protein expressions of Bax and Caspase-3,P<0.01; while increased ±dp/dtmax, elevated protein expression of Bcl-2 and the ratio of Bcl-2/Bax, P<0.01.
Conclusion: Fosinopril may inhibit myocardial cell apoptosis which occurred during CHF, by up-regulating Bcl-2 expression and down-regulating expressions of Bax and Caspase-3 in CHF rats, therefore improve the cardiac function.
