Expression of RIG-Ⅰgene in peripheral blood of patients with chronic hepatitis C and its ;correlations with hepatitis C virus load and typeⅠinterferon gene expression
10.3760/cma.j.issn.1674-2397.2016.01.004
- VernacularTitle:慢性丙型肝炎患者外周血RIG-Ⅰ基因表达与丙型肝炎病毒载量及Ⅰ型干扰素基因的相关性
- Author:
Fangping WANG
;
Pingan ZHANG
;
Zhili NIU
- Publication Type:Journal Article
- Keywords:
Hepatitis C,chronic;
Retinoic acid inducible gene Ⅰ;
Interferon type Ⅰ;
Polymerase chain reaction
- From:
Chinese Journal of Clinical Infectious Diseases
2016;(1):19-23
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of retinoic acid-inducible gene Ⅰ ( RIG-Ⅰ) in peripheral blood mononuclear cells (PBMCs) of patients with chronic hepatitis C (CHC), and to explore its correlations with type Ⅰinterferon gene expression and serum level of hepatitis C virus .Methods A total of 101 na?ve patients with CHC were enrolled from Renmin Hospital of Wuhan University during July 2014 and July 2015, and 69 healthy subjects served as controls .The expressions of RIG-Ⅰ, IFNαand IFNβmRNA in PBMCs and serum level of HCV RNA were detected by real-time quantitative polymerase chain reaction.Pearson correlation analysis was performed to investigate the correlations of RIG-Ⅰ mRNA expression with IFNα/βmRNA expression and serum level of HCV RNA .Results The relative expressions of RIG-Ⅰ, IFNα, IFNβmRNA to healthy controls in CHC group were 0.082, 0.022 and 0.156, respectively (t=7.83, 3.65 and 2.13, all P<0.05).RIG-Ⅰ mRNA expression in patients with HCV loads <(1 ×106 ) IU/mL was 1.79 times higher than that in patients with HCV loads≥(1 ×106 ) IU/mL (t=2.60, P <0.05).Pearson correlation analysis showed that the expression of RIG-Ⅰ mRNA was positively correlated with that of IFNαmRNA and IFNβmRNA (r=0.247 and 0.489, P<0.05), but not correlated with HCV load (r=0.187, P>0.05).Conclusion Expression of RIG-ⅠmRNA decreases in patients with CHC , and is positively correlated with that of IFNαand IFNβmRNA.
