Effect of Acupuncture on Cx43 Protein Expression in the Hippocampal Region in Rats with Acute Cerebral Infarction
10.13460/j.issn.1005-0957.2016.01.0090
- VernacularTitle:针刺对急性脑梗死大鼠海马区Cx43蛋白表达的影响
- Author:
Kun LIU
;
Jiangong HU
;
Liancheng ZHANG
;
Zhenxin XIAO
;
Yanying PAN
;
Baowang YANG
;
Yan MA
;
Fubin GUO
;
Zhankui WANG
- Publication Type:Journal Article
- Keywords:
Acupuncture therapy;
Cerebral infarction;
Reperfusion injury;
Cx43;
Rats;
Electroacupuncture
- From:
Shanghai Journal of Acupuncture and Moxibustion
2016;(1):90-93
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of acupuncture on gap junction protein Cx43 expression in the hippocampal region in ischemia/reperfusion rats.Methods Wistar rats were randomized into normal, model, non-point acupuncture and acupuncture groups. A rat model of focal cerebral ischemia/reperfusion was made by modified middle cerebral artery thread occlusion. The model, non-point acupuncture and acupuncture groups were separately divide into four subgroups: 30, 60, 180 and 360 min ischemia/reperfusion, 10 rats each. The middle and lateral lines of vertex were given electroacupuncture in the acupuncture group of rats. A subcostal fixed point 10 mm above the iliac crest on the affect side was selected as an acupuncture point in the non-point acupuncture group. The model group was not treated. Cx43 protein expression was determined by an immunohistochemical method.Results There was a statistically significant difference in the behavior disorder (Bederson’s) score between the acupuncture group and the non-point acupuncture or model group after different times of cerebral ischemia/ reperfusion (P<0.05). There was a statistically significant difference in hippocampal Cx43 content between model, acupuncture or non-point acupuncture group and the normal group and between the acupuncture group and the model or non-point acupuncture group after different times of cerebral ischemia/reperfusion (P<0.05).Conclusions Acupuncture can inhibit the overexpression of Cx43, intervene in cerebral ischemia-reperfusion injury and produce a neuroprotective effect in ischemic brain injury.
