On hemolysis of extracts of Lonicera Macranthoides flower bud and two saponins
10.3969/j.issn.1001-1978.2016.01.010
- VernacularTitle:灰毡毛忍冬花蕾提取物及其两种主要皂苷的溶血性研究
- Author:
Hongjin WANG
;
Hongyu WANG
;
Ran HE
;
Junjie WU
;
Xiaoyu XU
- Publication Type:Journal Article
- Keywords:
flower bud of Lonicera macranthoides;
MacranthoidinB;
DipsacosideB;
hemolysis;
visual in-spection;
spectrophotometry test
- From:
Chinese Pharmacological Bulletin
2016;(1):43-48
- CountryChina
- Language:Chinese
-
Abstract:
Aim To figure out whether Lonicera mac-ranthoides could induce hemolysis. Methods In vitro, macroscopic observation and spectrophotometry were used to observe whether the solutions of extracts from Lonicera macranthoides, MacranthoidinB and Dipsa-cosideB could induce hemolysis in 2% red cell suspen-sion of New Zealand white rabbits. And the three test-ed materials were prepared in concentration gradient of 5, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100 mg· L - 1; and in vivo, mice were respectively treated with MacranthoidinB (0. 110 g·kg - 1 , 0. 055 g·kg - 1 ), DipsacosideB(0. 020 g·kg - 1 , 0. 010 g·kg - 1 ), ex-tracts (2. 275 g·kg - 1 , 1. 137 g·kg - 1 , crude drugs) once per day for 7 days, and all of the tested doses de-pended on the clinical doses. Then, RBC, RET and MCHC before and after administration were tested. Re-sults The hemolytic ratio in each treated group was below 5% in vitro. And in vivo, the three materials did not induce hemolysis and had no significant influence on RBC,RET and MCHC(P > 0. 05). Conclusions Extracts from flower bud of Lonicera macranthoides, MacranthoidinB and DipsacosideB, have not caused hemolysis in vivo and in vitro in this research.
