Preliminary of research of effect of artesunate on invasion of human colon cancer Lovo cells
10.3969/j.issn.1001-1978.2016.01.013
- VernacularTitle:青蒿琥酯对人大肠癌 Lovo 细胞侵袭影响的初步研究
- Author:
Ying GUO
;
Jianhua GUO
;
Hang FU
;
Zhiyu TIAN
;
Qiang LUO
;
Fang LIU
;
Linxi ZHANG
- Publication Type:Journal Article
- Keywords:
artesunate;
colon cancer;
proliferation;
invasion;
HMGB1;
MMP-2
- From:
Chinese Pharmacological Bulletin
2016;(1):60-63
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of artesunate on the invasion of human colon cancer Lovo cells and the possible mechanisms. Methods After Lovo cells were treated with different doses of artesunate(20,80, 160 μmol·L - 1 ), the soft agar colony formation test was adopted to observe the anchorage-independent pro-liferation of Lovo cells. Transwell assay was used to determine the effect of artesunate on the invasion abili-ty of Lovo cells. And the protein expressions of HMGB1 and MMP-2 were investigated by western blot. Results Artesunate could significantly inhibit both proliferation and invasion ability of Lovo cells in a dose-dependent manner(P < 0. 01). The experimental group treated with artesunate significantly down-regula-ted the protein expressions of HMGB1 and MMP-2 compared with control group(P < 0. 05). Conclusion Artesunate could inhibit the invasion of human colon cancer Lovo cells by down-regulating HMGB1 and MMP-2 expressions.
