Inhibitory effects of eplerenone on cell proliferation via down-regulated SGK-1 pathway in rats with unilateral ureteral obstruction
10.3969/j.issn.1001-1978.2016.01.015
- VernacularTitle:依普利酮下调 SGK-1表达抑制梗阻性肾病细胞增殖的研究
- Author:
Limin WU
;
Lixiang CHEN
;
Lijuan LIANG
;
Zheng WANG
;
Miao WANG
;
Shaowei LIU
;
Yunzhao XIONG
;
Xuan WANG
;
Qingyou XU
- Publication Type:Journal Article
- Keywords:
eplerenone;
renal interstitial fibrosis;
cell proliferation;
UUO;
PCNA;
SGK-1;
TGF-β1
- From:
Chinese Pharmacological Bulletin
2016;(1):69-73
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the effect of mineralocor-ticoid receptor blockade eplerenone on cell proliferation in obstructed kidney of rats. Methods Renal intersti-tial fibrotic animals were made with unilateral ureteral obstruction (UUO) and treated with eplerenone100 mg · kg - 1 · d - 1 . The kidneys were harvested on the 10th day and proliferating cell nuclear antigen ( PC-NA ), serum and glucocorticoid induced kinase-1 (SGK-1 ) and transforming growth factor-β1 ( TGF-β1 ) were detected with immunohistochemistry and Western blot. Results Renal histopathology showed large quantities extracellular matrix (ECM) accumula-tion in kidney with UUO, large numbers of inflammato-ry cells infiltrated in renal interstitium, renal tubular expansion and exfoliation of epithelial cells . The cell proliferation and ECM accumulation were inhibited in eplerenone treated rats significantly. Immunohisto-chemistry and Western blot showed that expressions of PCNA,SGK-1 and TGF-β1 were significantly up-regu-lated with UUO and down-regulated by eplerenone. Conclusion Eplerenone plays the role in inhibiting the cell proliferation and reducing ECM accumulation by down-regulating expression of SGK-1 pathway in rats with unilateral ureteral obstruction.
