Determination of 1,8-TMP rhein and its pharmacokinetics in rat plasma by HPLC
10.3969/j.issn.1001-1978.2016.01.023
- VernacularTitle:HPLC 测定大鼠血浆中1,8-二川芎嗪基大黄酸及其药代动力学研究
- Author:
Zhaoliang PENG
;
Jie LI
;
Ling FAN
;
Xueqi WANG
;
Peng HUANG
;
Jincai LI
;
Dianlei WANG
;
Yajun CHEN
;
Shujun WANG
;
Shanshan WANG
;
Yue ZHANG
- Publication Type:Journal Article
- Keywords:
1,8-TMP Rhein;
HPLC;
rat;
pharmaco-kinetics;
plasma concentration;
intravenous injection
- From:
Chinese Pharmacological Bulletin
2016;(1):109-113
- CountryChina
- Language:Chinese
-
Abstract:
Aim To develop a HPLC method for the determination of the concentration of 1,8-TMP rhein in rat plasma and study the pharmacokinetics of 1,8-TMP rhein in rat plasma after single dose i. v. administration of 1,8-TMP rhein (2, 4, 8 mg·kg - 1 ). Methods Emodin was used as an internal standard. Plasma sam-ples were extracted with methanol and analyzed by HPLC. The mobile phase was methanol - 0. 1% for-mic acid water (78 ∶ 22, V/ V), with a flow rate of 1. 0 mL·min - 1 and UV 275 nm as the detection wave-length. The plasma concentration of 1,8-TMP rhein in rats was determined by HPLC after single-dose intrave-nous injection in rats with 2,4 and 8 mg·kg - 1 of 1,8-TMP rhein, and the pharmacokinetic parameters were caclulated by DAS 2. 1. Results The result of cali-bration curve was linear over the range of 0. 05 ~ 10. 00 mg·L - 1 (r = 0. 996 2). The lower limit of quantifica-tion was 0. 05 mg · L - 1 . The intra-day and inter-day precision (RSD% ) were both lower than 6% , and the extraction recoveries were higher than 88% , respec-tively. The validated method was successfully applied to a pharmacokinetic study after i. v. administration of 1,8-TMP rhein in rats with a dose of 2,4 and 8 mg· kg - 1 . The T1 / 2 was (68. 35 ± 1. 36), (69. 32 ± 2. 1) and (69. 32 ± 2. 03) min, respectively. The AUC0 - t was ( 101. 03 ± 24. 90 ), ( 144. 79 ± 3. 29 ) and (231. 92 ± 19. 30 ) min · mg · L - 1 , respectively. Conclusion A simple and specific HPLC method for the analysis of 1,8-TMP rhein is successfully developed and applied to a pharmacokinetic study in rat plasma.
