Model establishment of xenotransplantation of human breast cancer in zebrafish embryos
10.3969/j.issn.1001-1978.2016.01.027
- VernacularTitle:人乳腺癌斑马鱼移植瘤模型建立
- Author:
Xiqiang CHEN
;
Liwen HAN
;
Ximin WANG
;
Rongchun WANG
;
Hairong HOU
;
Kechun LIU
;
Weibing PENG
;
Chen SUN
;
Jian HAN
- Publication Type:Journal Article
- Keywords:
zebrafish;
xenograft;
breast cancer;
angiogenesis;
inhibitor;
tofacitinib
- From:
Chinese Pharmacological Bulletin
2016;(1):128-132
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the modeling of breast cancer in zebrafish embryos and its related protein expression. Methods 48hpf wild type AB/ TG(Transgenic) zebrafishs were micro-in-jected with breast cancer cell line: MCF-7,T-47D, MDA-MB-231 respectively, the relationship between the number of tumor and model application was investigated, and the number of sub-intestinal veins(SIVs) was detected under confocal microscope, as well as the metastasis of tumor cells in embryos; then the ze-brafish xenografts of MB-231 were co-cultured with tofacitinib/ptk787 for 48 h, optical density(OD) of the cell survival and subintestinal veins(SIVs) were evaluated under confocal micro-scope, and Western blot(WB) analysis was used to test micro-circumstances related protein. Results When the number of in-oculated cells was more than 200 per embryo, xenograft model rate woule be more than 0. 90;MB-231 xenografts showed metas-tasis feature in zebrafish, which could be inhibited by tofacitinib (P < 0. 01), while the number of xenograft MB-231 cells was reduced significantly(P < 0. 01); in another zebrafish xenografts SIVs assay, the tumor could promote the proliferation of SIVs, and 4 mg·L - 1 PTK787 showed inhibiton effect( P < 0. 01). Western blot showed 4d T-47D xenograft zebrafish got more HER2 expression than AB embryos; VEGFa expression in ze-brafish MB-231 model group was higher, and model zebrafish P53 expressi was higher after treated by tofacitinib. Conclusion A zebrafish xenograft model of human brest cancer can be es-tablished, which demonstrates applicability for screening com-pounds in drug discovery studies.
