Effect of different tumescent local anesthesia on the proliferation and differentiation of human adipose stem cells culturedin vitro
10.3969/j.issn.2095-4344.2015.50.006
- VernacularTitle:不同局部肿胀麻醉液对体外培养人脂肪干细胞增殖与成脂分化的影响
- Author:
Zhigang WANG
;
Jianping WU
;
Wenxiang ZHAO
- Publication Type:Journal Article
- Keywords:
Stem Cels;
Adipose Tissue;
Anesthetics,Local;
Cel Proliferation;
Adipogenesis;
Tissue Engineering
- From:
Chinese Journal of Tissue Engineering Research
2015;(50):8067-8071
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Different local anesthetic solutions for tumescent liposuction are necessary and have a certain effect on the biological characteristics of human adipose stem cels.
OBJECTIVE:To explore the effect of different tumescent local anesthesia on the proliferation and differentiation of human adipose stem cels culturedin vitro.
METHODS: Passage 3 human adipose stem cels were intervened by different tumescent local anesthesia (bupivacaine, ropivacaine, lidocaine). Cels cultured in low-glucose DMEM served as controls. Proliferation ability, lactate dehydrogenase activity in the supernatant and percentage of cels under adipogenic differentiation were detected.
RESULTS AND CONCLUSION:After intervention for 1, 2, 3, 4, 5 days, the supernatant lactate dehydrogenase activity in the bupivacaine, ropivacaine, lidocaine groups was significantly higher than that in the control group (P < 0.05), and moreover, the lactate dehydrogenase activity in the bupivacaine group was significantly higher than that in the ropivacaine group and lidocaine group (P < 0.05). The absorbance value of human adipose stem cels was lower in the bupivacaine, ropivacaine, lidocaine groups than the control group (P < 0.05) as wel as lower in the bupivacaine group than the ropivacaine and lidocaine groups (P < 0.05). There was no significant difference in the percentage of cels under adipogenic differentiation between groups (P > 0.05). Overal, these findings indicate that different tumescent local anesthesia (bupivacaine, ropivacaine, lidocaine) can al inhibit the proliferation of human adipose stem cels, but have no certain effects on the adipogenic differentiation.
