Differentiation of neural stem cells induced by glial cell line-derived neurotrophic factor
10.3969/j.issn.2095-4344.2015.50.023
- VernacularTitle:转染胶质细胞源性神经营养因子诱导神经干细胞分化
- Author:
Jia HE
;
Bo YAN
- Publication Type:Journal Article
- Keywords:
Neural Stem Cels;
Genes;
Glial Cel Line-Derived Neurotrophic Factor;
Cel Differentiation;
Tissue Engineering
- From:
Chinese Journal of Tissue Engineering Research
2015;(50):8167-8171
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:The use of neural stem cels provides a new approach for nervous system functional reconstruction and nerve regeneration. How to solve the induced differentiation of neural stem cels is stil a research hotspot.
OBJECTIVE:To investigate the differentiation of rat neural stem cels into neurons and dopaminergic neurons under induction of glial cel-line derived neurotrophic factor gene.
METHODS:PcDNA3-GDNF-GFP plasmids were constructed and transferredvia lipidosome into rat embryonic neural stem cels. Differentiation of neural stem cels after transfection was identified under a fluorescence microscope. Immunofluorescent staining was used to detect expression of β-tubulin III and tyrosine hydroxylase.
RESULTS AND CONCLUSION:At 3 days after transfection, green fluorescence was observed in the transfected cels that were globular. At 7 days after transfection, the proportion of neural stem cels differentiating into neurons and dopaminergic neurons were significantly increased. These results indicate that the glial cel-line derived neurotrophic factor gene can induce the differentiation of neural stem cels into neurons and dopaminergic neurons.
