Effect of human placenta-derived mesenchymal stem cells on cytotrophoblast apoptosis in gestational hypertension:study protocol for a randomized controlled trial
10.3969/j.issn.2095-4344.2015.50.027
- VernacularTitle:人胎盘来源间充质干细胞对妊娠期高血压病细胞滋养细胞凋亡的影响:随机对照实验方案
- Author:
Li ZHANG
;
Zhi LI
;
Jing WANG
;
Wei LIU
- Publication Type:Journal Article
- Keywords:
Stem Cels;
Hypertension,Pregnancy-Induced;
Placenta;
Stem Cel Transplantation;
Apoptosis;
Tissue Engineering
- From:
Chinese Journal of Tissue Engineering Research
2015;(50):8190-8194
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Excessive apoptosis and decreased infiltration of cytotrophoblasts are essential causes for hypertension in pregnancy. Human placenta-derived mesenchymal stem cels contribute to damage repair, which has been shown in many studies, and moreover, human placenta-derived mesenchymal stem cels have a certain endocrine function and can act on the other tissues in an autocrine or paracrine manner. Therefore, we attempt to explore whether the human placenta-derived mesenchymal stem cel can repair damaged cytotrophoblasts, and then to gestational hypertension.
METHODS/DESIGN:This is a randomized controled cytological experiment. Human placenta-derived mesenchymal stem cel culture medium is colected and filtrated as human placenta-derived mesenchymal stem cel conditioned medium. Human cytotrophoblasts, JEG-3 cels, are cultured and randomized into three groups:15% normal pregnant serum is added in control group; 15% serum from severe pre-eclampsia patients is added in gestational hypertension model group; and in condition medium group, 15% serum from severe pre-eclampsia patients is added for 24 hours of culture, and then human placenta-derived mesenchymal stem cel conditioned medium containing 15% serum from severe pre-eclampsia patients is used instead. Flow cytometry is used to detect cel apoptosis rate.
DISCUSSION: This study wil help to find the feasibility of cel transplantation for gestational hypertension by exploring the effect of human placenta-derived mesenchymal stem cels on cytotrophoblasts function in gestational hypertension.
ETHICAL APPROVAL: The protocol is approved by the Ethics Committee of Shenyang Central Hospital of Shenyang Medical University. Informed consent is obtained from normal and pre-eclampsia women in pregnancy.