Determining the content of naringin and neonaringin in Biantong Capsule by HPLC
- VernacularTitle:高效液相色谱法测定便通胶囊中柚皮苷和新橙皮苷的含量
- Author:
Yongliang WANG
;
Yan DOU
- Publication Type:Journal Article
- Keywords:
Biantong Capsule;
naringin;
neonaringin;
HPLC
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;(12):174-176
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method for the determination of naringin and neonaringin in Biantong Capsule .Methods The HPLC analysis was performed on a Zorbax Extend C18Column(250 mm ×4.6 mm,5μm), Acetonitrile-0.1 % phosphoric acid (20:80)as mobile phase, the detective wavelength was at 283 nm, flow rate was 1.0 mL/min, the column temperature was 30℃.Results Naringin and neohes peridin showed a good linear relationship with range in 0.1147-2.867μg, 0.0807-2.0169μg, respectively.The recovery were 98.7 %and 98.4 %.Conclusion The method is simple, rapid, accurate and reliable.And it can be applied to control the quality of Biantong Capsule.