Determination of anthraquinones in Niuhuang Qinghuo Pills by HPLC
- VernacularTitle:高效液相色谱法测定牛黄清火丸中蒽醌类化合物的含量
- Author:
Quanping DIAO
;
Hua GUO
;
Linlin LV
;
Tiechun LI
;
Dongyan HOU
- Publication Type:Journal Article
- Keywords:
Niuhuang Qinghuo Pills;
anthraquinones;
HPLC
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;(12):177-179
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the method of determination for rhein, emodin, chrysophanol and physcion in Niuhuang Qinghuo Pills. Methods Sample was hydrolyzed by 20% H2 SO4 , and anthraquinones were extracted by soxhlet extraction with acetone as solvent, and were determined by HPLC with Kromasil C18 as column, methanol-0.5%H3 PO4 (75:25,V/V) as mobile phase at the flow of 1.0 mL/min, 230 nm as the detection wavelength.Results The linear relationship of rhein, emodin, chrysophanol and physcion were 1.50-150, 0.950-95.0, 1.30-130 and 1.15-115 mg/L. The anthraquinones were seperated completely, the recovery of 4 anthraquinones were 98.14% -101.3%.Conclusion This method is simple, accurate, steady, and could be used for the quality control of Niuhuang Qinghuo Pills.
