Establishment of a universal fluorescent multiplex cPCR method for detection of copy number variations in mice
- VernacularTitle:构建基于荧光多重 cPCR 的小鼠基因拷贝数检测方法
- Author:
Tianzhu CHAO
;
Pengxiang LI
;
Fuyi XU
;
Kai LI
;
Yuxun ZHOU
;
Junhua XIAO
- Publication Type:Journal Article
- Keywords:
Copy number variations,CNVs;
Competitive template;
Universal fluorescence primers;
cPCR;
Mice
- From:
Acta Laboratorium Animalis Scientia Sinica
2015;(6):591-596
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a high throughput general multiple competitive polymerase chain reaction ( cPCR) detecting method of copy number variations ( CNVs) for the population of chromosome 1 substitution strains from wild mice.Method The selected 14 loci, including 11 CNVs on chromosome 1 and internal control loci on other three chromosmes (Chr 7, Chr 19 and Chr X), were detected based on the universal fluorescent primer multiple competitive pol-ymerase chain reaction.All specific cloned plasmids were constructed as competitors.Results Altogether 11 CNVs were designed in one panel, and the copy of Chr X accurately reflects the gender.Conclusions A rapid and high-throughput fluorescent multiplex cPCR assay is established which can be used for detection of copy number variations on chromosome 1 in mice.
