Effect of alcohol extract of Coreopsis tinctoria Nutt on intestinal flora in type 2 diabetic mice
10.3969/j.issn.1005-4847.2015.06.016
- VernacularTitle:两色金鸡菊醇提物对2型糖尿病小鼠肠道菌群的影响
- Author:
Kainan ZHANG
;
Mei LONG
;
Xinjian RAN
;
Ying YANG
;
Lei TONG
;
Qingcheng LI
;
Jianhui XIONG
;
Xinmin MAO
;
Liang TAO
;
Linlin LI
- Publication Type:Journal Article
- Keywords:
Clostridium coccoides;
Bacteroides thetaiotaomicron;
Type 2 diabetes mellitus:Real-time PCR
- From:
Acta Laboratorium Animalis Scientia Sinica
2015;(6):628-633
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the differences in intestinal flora of normal and type 2 diabetic mice, the effect of alcoholic extract of Coreopsis tinctoria Nutt on mouse intestinal flora, and explore the possible relationship between alcoholic extract of Coreopsis tinctoria Nutt, intestinal flora and type 2 diabetes mellitus (T2DM) in mice.Methods Stool samples were collected from the normal control group (A), high dose (1.8 g/kg) (B) and moderate dose (1.2 g/kg) (C) alco-holic extract of Coreopsis tinctoria Nutt model groups, metformin (0.2 g/kg) treatment group (D) and blank control (E) group.16S rDNA real-time quantitative PCR assay was used to determine the levels of Clostridium coccoides and Bacteroides thetaiotaomicron in the stool samples.Pearson analysis method was used to analyze the correlation between the levels of tar-get bacterial species and the fasting blood glucose ( FBG) in the mice.Results 1.Compared with the normal control group, the levels of Clostridium coccoides and Bacteroides thetaiotaomicron in the T2DM model group were significantly low-ered (P=0.017, P=0.002).2.Compared with the model group, the levels of Clostridium coccoides and Bacteroides the-taiotaomicron of the high dose Coreopsis tinctoria Nutt alcohol extract group were significantly different ( 2 weeks: P =0.027, P=0.006;4 weeks:P=0.007, P=0.012).3.The levels of Clostridium coccoides and Bacteroides thetaiotaomi-cron were positively correlated with the FBG level in the mice.Conclusions The alcohol extract of Coreopsis tinctoria Nutt has certain effect on the intestinal flora in type 2 diabetic mice and there is certain correlation between the effect of alcohol extract of Coreopsis tinctoria Nutt and their blood glucose level.
