Effects of perilla oil on the insulin sensitivity-related gene expression in skeletal muscle in insulin resistant rats
10.3969.j.issn.1671-7856.2015.12.001
- VernacularTitle:苏子油对胰岛素抵抗大鼠骨骼肌胰岛素敏感性相关基因表达的影响
- Author:
Xianxing XIE
;
Tao ZHANG
;
Shuang ZHAO
;
Xinglan AN
;
Lanzhi MA
;
Ming DING
;
Yuan LIU
- Publication Type:Journal Article
- Keywords:
α-Linolenic acid;
Insulin resistance;
Rats;
Glucose transporter 4;
Insulin receptor substrate-1
- From:
Chinese Journal of Comparative Medicine
2015;(12):1-5
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study aims to investigate the effects of high-fat diet rich in perilla oil on the insulin sensitivity-related gene expression in skeletal muscle in insulin resistant rats.Methods The insulin resistant ( IR) rat models were randomly divided into 2 groups, including high fat group ( HF) and perilla oil ( PO) intervention group fed with 20%substitution of lard energy in the HF.The insulin sensitivity of rats was measured after 4 weeks.Theα-linolenic acid ( ALA) content of PO in the rat plasma were analyzed by gas chromatograph.Real-time PCR was applied to measure glucose transporter 4 ( GLUT4 ) and insulin receptor substrate-1 ( IRS-1 ) mRNA, and Western blot assay was used for detecting the expression of GLUT4 and IRS-1 in the skeletal muscle.Results At the gene and protein levels, PO remarkably reduced the level of IRS-1 and upregulated the level of GLUT4 with increasing intake of ALA and serum ALA content in IR rats.The results of hyperinsulinemic-euglycemic clamp test showed no significant difference between the two groups.Conclusions The results of our study suggest that consumption of n-3 PUFA at levels that can typically be found in the diet fed to IR rats in the form of ALA (0.556 g/d) may not improve insulin sensitivity, even though regulating the expression of GLUT4 and IRS-1 in the skeletal muscle.
