Clinicopathological Significance and Relevant Molecular Mechanisms of Beclin 1 in Gastric Cancer
- VernacularTitle:Beclin 1表达在胃癌发生和演进中的临床病理意义及作用机制
- Author:
Huachuan ZHENG
;
Daofu SHEN
;
Xuefeng YANG
;
Shuai SHI
;
Yang GAO
;
Shuang ZHAO
;
Wenlu ZHANG
- Publication Type:Journal Article
- Keywords:
gastric cancer;
Beclin 1;
pathogenesis;
progression;
prognosis
- From:
Journal of China Medical University
2015;(12):1061-1065
- CountryChina
- Language:Chinese
-
Abstract:
Objective to explore the role of Beclin 1 in gastric carcinogenesis and subsequent progression. Methods MkN28 cells were trans-fected with Beclin 1-expressing plasmid,and then the proliferation and cell cycle was measured by CCk-8 and PI staining. Beclin 1 expression was examined by immunohistochemistry and in situ hybridization on tissue microarrays containing gastric cancers,adjacent non-neoplastic mucosa,and metastatic lymph node. the correlation with the tumorgenesis,clinicopathological and prognostic parameters was analyzed. Results Beclin 1 overex-pression resulted in G2 arrest of MkN28 cells and reduced the proliferation. Beclin 1 mRNA was highly expressed in gastric cancer than matched mu-cosa by ISH(P < 0.05). Beclin 1 was highly expressed in male than female patients with gastric cancer(P < 0.05). the elder patients with gastric cancer had higher Beclin 1 expression than younger ones(P < 0.05). the diffuse-type carcinomas showed less Beclin 1 expression than intestinal and mixed type ones(P < 0.05). kaplan-Meier analysis indicated that Beclin 1 expression was positively correlated to favorable prognosis of the can-cer patients(P < 0.05). Conclusion Beclin 1 expression is closely linked to pathogenesis,metastasis and differentiation of gastric cancer. Beclin 1 might be employed to indicate the favorable prognosis of gastric cancer patients and regarded as a target of gene therapy.
