The effect on proliferation and invasion after silence c-cbl gene in ovarian cancer SKOV3 cell
10.3969/j.issn.1671-8348.2015.36.004
- VernacularTitle:c-cbl 基因沉默对卵巢癌 SKOV3细胞增殖和侵袭的影响
- Author:
Chong FU
;
Jianguo HU
- Publication Type:Journal Article
- Keywords:
c-cbl;
ovarian neoplasms;
cell proliferation;
neoplasm invasiveness
- From:
Chongqing Medicine
2015;(36):5061-5064
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of c-cbl protein in ovarian cancer and its effect on proliferation and invasion in ovarian cancer cell .Methods The expression of c-cbl protein in ovarian tissue were detected by immunohistochemical(S-P meth-od) ,the mRNA expression of c-cbl protein in ovarian cancer SKOV3 cells were detected .the proliferation ability and migration in o-varian cancer SKOV3 cells were detected by EdU assay and Transwell assay .Western blot was used to detect the expression of P21 and P53 protein .Results c-cbl was located in the cytoplasm in ovarian cancer tissues .c-cbl showed moderate or strong positive ex-pression in ovarian cancer .In contrast ,it showed weakly positive or negative expression in normal ovarian tissue .c-cbl expressed in-creased significantly in ovarian cancer compared with those of in normal ovarian tissue(P < 0 .05) ;c-cbl expressed was associated with FIGO stage in ovarian cancer(P< 0 .05) ;silence c-cbl expression in ovarian cancer SKOV3 cells decreased the proliferation and invasion(P< 0 .05) .Silence c-cbl expression increased the expression of P21 and P53 protein(P< 0 .05) .Conclusion c-cbl was in-creased expression in ovarian cancer tissues .Silence c-cbl expression may be related to increased expression of P21 and P53 .
