The correlation study of the glycogene ST8SIA4 in the multidrug resistance of human chronic myeloid leukemia
10.3969/j.issn.1671-8348.2015.36.008
- VernacularTitle:糖基因 ST8SIA4在人慢性粒细胞白血病多药耐药中的作用研究
- Author:
Zhaohai ZHANG
;
Ruihong ZHANG
- Publication Type:Journal Article
- Keywords:
glycogene ST8SIA4;
multidrug resistance;
myeloid leukemia,chronic
- From:
Chongqing Medicine
2015;(36):5074-5076
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the differential expression of ST8SIA4 in the both chronic myeloid leukemia(CML) cell lines KCL22 and adriamycin resistant cell lines KCL22/ADR ,peripheral blood mononuclear cells (PBMC) of CML patients ,to clar-ify the correlation of ST8SIA4 and multidrug resistance of CML .Methods Using real-time PCR and Western blot for quantification of ST8SIA4 ,we compared the differential expression of ST8SIA4 in CML cell lines and PBMC of CML patients .The differentially expressed ST8SIA4 gene was silenced ,and the chemosensitivity to anti-tumor drugs was analyzed in vitro .Results ST8SIA4 was differentially expressed in CML cell lines and PBMC of CML patients(P< 0 .05) .The altered level of ST8SIA4 was increased drug-resistant phenotype of KCL22/ADR cells both in vitro and in vivo(P< 0 .05) .Conclusion The expression of ST8SIA4 was different in CML cell lines and PBMC of CML patients ,these characteristic alterations were associated with multidrug resistance in CML .
