The clinical study on the relationship between mechanism of miRNA-126-3p and pulmonary hypertension
10.3969/j.issn.1671-8348.2015.36.018
- VernacularTitle:miRNA-126-3p 与先天性心脏病肺动脉高压发病机制相关性的临床研究
- Author:
Li CHENG
;
Xiaoqing LI
;
Wenjiang CHEN
;
Can CHEN
- Publication Type:Journal Article
- Keywords:
miRNA-126-3p;
hypertension,pulmonary;
vascular endothelial growth factor A;
signaling pathways
- From:
Chongqing Medicine
2015;(36):5103-5106
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the corelation between miRNA-126-3p and pathogenesis of pulmonary hypertension . Methods Totally 25 patients with congenital heart disease ,including 11 cases with pulmonary hypertension and 14 control cases , were recruited .The expression of miRNA-126-3p was detected by qRT-PCR ,and we used starBase to predict the biological informa-tion miRNA-126-3p ,and then verified it by the level of mRNA and protein .Results There was no statistical difference in age ,gen-der ,and biochemical examination(P> 0 .05) ;the expression of miRNA-126-3p in the pulmonary hypertension cases was significantly higher(P< 0 .01) ;starBase found that miRNA-126-3p may participate in binding protein ,signal transduction ,cell differentiation and regulation of cell morphology ,the regulation of MAPK and insulin receptor signaling pathways ,etc .Its target genes mainly contain VEGFA ,SPRED1 ,PIK3R2 ,etc ;the mRNA and protein level of VEGFA in PH group were statistically significantly than control group(P< 0 .01) ;the miRNA-126-3p and VEGFA showed positive correlation(P< 0 .01) .Conclusion miRNA-126-3p may partici-pate in the occurrence of pulmonary hypertension by regulating VEGFA .
