Study on the electrochemical immunosensor for ultrasensitive detection of myeloperoxidase
10.3969/j.issn.1671-8348.2015.36.020
- VernacularTitle:电化学免疫传感器超灵敏检测髓过氧化物酶的研究
- Author:
Lingsong LU
;
Bei LIU
;
Xiao MA
;
Cheng YU
;
Shun WU
;
Jianhang LENG
- Publication Type:Journal Article
- Keywords:
myelo peroxidase;
electrochemical immunosensor;
graphitized mesoporous carbon nanoparticles;
Au nanoparticles
- From:
Chongqing Medicine
2015;(36):5109-5111
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct an electrochemical immunosensor for ultrasensitive determination of myeloperoxidase (MPO) .Methods The electrochemical immunosensor for M PO was prepared by modifying the electrode using Au-graphitized me-soporous carbon nanoparticles(AuNPs@ GMCs) hybrid and immobilizing MPO antibodies onto the glass carbon electrode surface . The effect of experimental parameters on the immunosensor and results comparison with ELISA were investigated .Results The immunosensor was sensitive to M PO with a linear relationship between 2 .000 and 300 .000 ng/mL and a correlation coefficient of 0 .999 ;the detection limit was 0 .5 ng/mL .The correlation coefficient of two methods was 0 .983 .Conclusion The immunosensor can be used for ultrasensitive detection of MPO .
