Regulation of mRNA expression of OPG and RANKL in hPDLCs by hypoxia in vitro
10.3969/j.issn.1671-8348.2015.35.018
- VernacularTitle:缺氧对人牙周膜细胞OPG和RANKL 基因表达的影响
- Author:
Aishu REN
;
Gang FU
;
Yu QIU
;
Ke HE
- Publication Type:Journal Article
- Keywords:
anoxia;
periodontal ligament;
osteoprotegerin;
receptor activator for nuclear factor-κB ligand
- From:
Chongqing Medicine
2015;(35):4955-4957
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of hypoxia on the mRNA expression of osteoprotegerin(OPG) and receptor activa‐tor for nuclear factor‐κB ligand(RANKL) in human periodontal ligament cells(hPDLCs) and to explore the role of hypoxia in the orthodontic bone resorption in pressure side .Methods The primary hPDLCs were cultivated with enzyme digestion assay and tis‐sue cultivation .The 3-5 generations of hPDLCs were respectively cultured 3 ,6 ,12 and 24 h in normoxia condition (20% O2 ) or in hypoxia condition (2% O2 ) .The mRNA expression of OPG and RANKL were detected with RT‐PCR .The experimental data was analyzed by one way ANOVA using SPSS15 .0 .Results After cultivated in hypoxia condition for 3 h or 6 h ,the mRNA expression of OPG and RANKL in hPDLCs didn′t change significant(P> 0 .05) .After cultivated in hypoxia condition for 12 h or 24 h ,the mRNA expression of OPG in hPDLCs decreased while the RANKL increased .Thus the ratio of RANKL/OPG increased and the difference was significant(P<0 .05) .Conclusion Hypoxia can regulate the mRNA expression of OPG and RANKL in hPDLCs and will promote the orthodontic bone resorption in pressure side .
