Effects of TSG on H2O2-induced Apoptosis and Expressions of XIAP and p53 in HUVECs
10.3870/j.issn.1672-0741.2015.06.003
- VernacularTitle:二苯乙烯苷对过氧化氢诱导内皮细胞凋亡及 XIA P和 p53表达的影响
- Author:
Ying YANG
;
Xiqiang GAO
;
Shiyin LONG
- Publication Type:Journal Article
- Keywords:
atherosclerosis;
TSG;
X-linked inhibitor of apoptosis protein;
p53
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2015;(6):629-633
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of tetrahydroxy stilbene glucoside(TSG)on H2O2‐induced apoptosis of human umbilical vein endothelial cells (HUVECs)and on the expressions of X‐linked inhibitor of apoptosis protein (XIAP)and p53.Methods HUVECs were cultured in vitro and divided into 6 groups:control group ,300 μmol/L H2O2 group ,1 μmol/L TSG+ 300 μmol/L H2O2 group ,10 μmol/L TSG+300 μmol/L H2O2 group ,100 μmol/L TSG+ 300 μmol/L H2O2 group ,30μmol/L Embelin+ 10 μmol/L TSG+ 300 μmol/L H2O2 group.The morphology of apoptotic cells was observed by Hoechst 33258 staining.The mRNA and protein expressions of XIAP ,p53 ,Caspase‐3 were detected by RT‐PCR and Western blotting , respectively.Results The number of apoptotic cells and the expression level of p53 were significantly increased while the ex‐pression level of XIAP was dramatically decreased in H2O2 group as compared with control group.The expression level of p53 and the number of apoptotic cells were down‐regulated while the expression level of XIAP was up‐regulated after treatment with 10 or 100 μmol/L TSG when compared with H2O2group.Moreover ,compared with those in 10 μmol/L TSGgroup ,the number of apoptotic cells and the expression of Caspase‐3 were significantly enhanced after pretreatment with 30 μmol/L Embelin for 6 h.Conclusion TSG can inhibit H2O2‐induced apoptosis of HUVECs by down‐regulating the expression level of p53 and up‐reg‐ulating the expression level of XIAP.
