Effects of Neuregulin-1 on Calcium Transients in Mouse Ventricular Myocytes
10.3870/j.issn.1672-0741.2015.06.007
- VernacularTitle:N eureg ulin-1对小鼠心室肌细胞钙瞬变的影响
- Author:
Qiong XIONG
;
Long WANG
- Publication Type:Journal Article
- Keywords:
neuregulin-1;
ventricular myocytes;
electrical stimulation;
Ca2+ transients
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2015;(6):652-655
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of neuregulin‐1 (NRG‐1)on calcium transients in mouse ventricular myo‐cytes.Methods Nine C57BL/6 mice of male were randomly dived into 3 groups :the blank control group ,NRG‐1 group ,and iso‐proterenol(ISO)group.Their hearts were removed and immediately cannulated via the aorta and retrogradely perfused with en‐zymatic isolation solution to get single ventricular cell by Langendorff system.Myocytes were loaded with the Ca2+ indicator Fluo‐4 and subjected to electrical field stimulation at 0.5 or 1 Hz by using living cells workstation.The change of fluorescene in‐tensity was recorded simultaneously in each group.Results Compared with the blank control group ,NRG‐1 group had signifi‐cantly increased Ca2+ transient amplitude ΔF/F0 (n=10 ,P<0.05) ,and reduced Ca2+ transient recovery time RT50 (n=10 ,P<0.05).There were no significant differences in Ca2+ transient amplitude ,Ca2+ transient recovery time and the time to peak be‐tween NRG‐1 and ISO groups(n=10 ,P>0.05).Conclusion NRG‐1 can increase Ca2+ transient amplitude and Ca2+ transients and reduce the time interval of Ca2+ transients.
