Effect of Ramipril and Trimetazidine on Renal Cell Apoptosis and Cytochrome C Expression in Experimental Rats With Chronic Heart Failure
10.3969/j.issn.1000-3614.2015.12.018
- VernacularTitle:雷米普利与曲美他嗪对慢性心力衰竭大鼠肾脏细胞凋亡及细胞色素C的影响
- Author:
Mengyun ZHANG
;
Xiaoou YANG
;
Yujuan PENG
;
Xiangyu FENG
;
Xiuhua LI
- Publication Type:Journal Article
- Keywords:
Ramipril;
Trimetazidine;
Heart Failure;
Apoptosis
- From:
Chinese Circulation Journal
2015;(12):1208-1211
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effects of ramipril, trimetazidine and the combination of ramipril and trimetazidine on renal cell apoptosis index (AI) and cytochrome C (Cyt-C) expression in experimental rats with chronic heart failure (CHF).
Methods: CHF model was established by partially banding of abdominal aorta superior to renal artery in experimental rats. A total of 50 male Wistar rats were randomly divided into 5 groups: Sham operation group, Model group, Ramipril group, Trimetazidine group and Combination (ramipril and trimetazidine) group.n=10 in each group. Renal tubular cell AI was examined by TUNEL method, mRNA and protein expressions of Cyt-C were detected by RT-PCR and Western Blot analysis in each group respectively.
Results: Compared with Sham operation group, Model group had increased AI of renal tubular cells, increased mRNA and protein expressions of Cyt-C,P<0.01. Compared with Model group, Ramipril group, Trimetazidine group and Combination group showed decreased AI of renal tubular cells (20.02 ± 1.14) %, (20.10 ± 1.2) % and (14.27 ± 1.40) % vs ( 40.82 ± 1.31) %; reduced Cyt-C mRNA expression (0.54 ± 0.06), ( 0.56 ± 0.05) and (0.44 ± 0.04) vs (0.89 ± 0.03); reduced Cyt-C protein expression (1.50 ± 0.11), (1.58 ± 0.12) and (0.75 ± 0.06) vs (2.53 ± 0.10); the most reduction was obtain by Combination group, allP<0.01.
Conclusion: Ramipril and trimetazidine can inhibit renal cell apoptosis and effectively improve the renal function in CHF rats. Combined medication is better than either of them alone.
