Effects of the Pup-proteasome system on the growth of Mycobacterium smegmatis strains

VernacularTitle:类泛素-蛋白酶体系统对耻垢分枝杆菌生长活力的影响
Author: Yi LIU ; Yu XUE ; Xuxia ZHANG ; Cong YAO ; Chuanyou LI
Publication Type:Journal Article
Keywords: Mycobacterium; Gene knockout; Prokaryotic ubiquitin-like protein; Proteasome; Via-bility
From:Chinese Journal of Microbiology and Immunology 2015;(11):832-835
CountryChina
Language:Chinese
Abstract: Objective To study the effects of prokaryotic ubiquitin-like protein ( Pup)-proteasome system on the growth of Mycobacterium strains.Methods The genes encoding Pup ( pup gene) and protea-someβsubunit ( prcB gene) were respectively knocked out from Mycobacterium smegmatis ( M.sm) strains by homologous recombination.The growth and viability of the wild-type and mutant strains of M.sm were an-alyzed under normal culture condition and under hypoxia as well as anaerobic conditions.Results The pup and prcB genes were completely and precisely knocked out from M.sm strains and the mutant strains were named △SM-Pup and△SM-prcB, respectively.The△SM-Pup strains grew faster than the wild type ( WT) and△SM-prcB strains.No significantly differences in the growth of M.sm were found between the WT and△SM-prcB strains.Conclusion The Pup-proteasome system was involved in the growth of M.sm, espe-cially the pup gene.There was difference between pup and prcB genes in regulating the growth of M.sm.The functions and influences of Pup-proteasome system still need further investigation.