Strong IgA-mediated mucosal immune responses elicited in BALB/c mice primed with Vaccinia Tian-tan-based HIV vaccine intramuscularly and boosted with protein intranasally
10.3760/cma.j.issn.0254-5101.2015.11.010
- VernacularTitle:重组痘苗病毒HIV疫苗肌注初免蛋白疫苗滴鼻加强后激发高强度黏膜IgA应答反应
- Author:
Fang LIU
;
Ying LIU
;
Rongmin WANG
;
Yiming SHAO
;
Ying LIU
;
Shuhui WANG
- Publication Type:Journal Article
- Keywords:
HIV Vaccine;
Vaccinia Tiantan strain;
HIV protein vaccine;
Mucoantibody
- From:
Chinese Journal of Microbiology and Immunology
2015;(11):840-845
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the mucosal immune responses induced in BALB/c mice after immunization with Vaccinia Tiantan-based HIV vaccine in combination with protein and to evaluate the effi-cacy of different immune strategies and adjuvants.Methods The BALB/c mice were intramuscularly or in-tranasally immunized with the recombinant Vaccinia virus Tiantan strain ( rTV) carrying CN54 Gag-Pol-Env gene and boosted with the gp140 protein and MF59 adjuvant by intramuscular, intranasal or subcutaneous in-jection.Serum, saliva and vaginal lavage samples were collected from the BALB/c mice.The titers of anti-gen specific IgG and IgA were detected by ELISA.Results Significantly enhanced mucosal immune respon-ses were induced by immunization with gp140 protein used in combination with MF59 adjuvant.The IgA an-tibodies elicited in mice mucosal tissues by gp140 protein and MF59 adjuvant were as high as those induced by gp140 protein and cholera toxin subunit B or recombinant flagellin adjuvant.High tiers of gp140-specific IgA antibodies were observed in serum, saliva and vaginal lavage samples from the mice intramuscularly primed with rTV and intranasally boosted with gp140 protein and MF59 adjuvant.The tiers of IgA antibodies in serum and mucosal tissue samples were 1 ∶15 000 and 1 ∶600, respectively.Conclusion High titers of mucosal IgA antibodies were elicited in BALB/c mice intramuscularly primed with Vaccinia Tiantan-based HIV vaccine and intranasally boosted with gp140 protein and MF59 adjuvant, especially in vaginal and oral tissues.This immunization strategy might be able to block the heterosexual transmission of HIV-1 through va-ginal and oral mucosa.
