Effects ofZi-Bu Pi-YinRecipe on Dendritic Spines in Spleen-YinDeficiency Dementia Model Rats
10.11842/wst.2015.10.013
- VernacularTitle:滋补脾阴方药对脾阴虚痴呆大鼠脑内树突棘变化影响的研究＊
- Author:
Hua SUI
;
Libin ZHAN
;
Zhenghong JI
;
Xiaoyang GONG
;
Jin GONG
;
Jian WANG
- Publication Type:Journal Article
- Keywords:
Spleen-yin deficiency dementia;
Zi-Bu Pi-YinRecipe;
dendritic spine;
Golgi staining
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(10):2029-2033
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to observe the changes of dendritic spine density in different regions of brain among spleen-yindeficiency dementia (SYDD) model rats, in order to investigate the effects ofZi-Bu Pi-Yin Recipe (ZBPYR) on dendritic spines. Spleen-yindeficiency (SYD) rats were modeled by classical method. And incubatedβ-Amyloid 1-40 (Aβ1-40) was injected into the hippocampus of each rat to make SYDD model, which received the administration of ZBPYR. Golgi staining was used to stain dendritic spine in different regions of brain in rat model for the observation of the amount and shape. The results showed that dendritic spine density in different regions of hippocampus and cortex in SYDD group was reduced than that of the SYD group. Compared with the dementia group and the SYDD group, the dendritic spine density in different regions of hippocampus and cortex of the SYDD + ZBPYR group was increased. Compared with the blank control group, the dendritic spine density in different regions of hippocampus and cortex in rats from the dementia group was reduced. It was concluded that there were different degrees of reducing in the dendritic spine density of different brain regions in SYDD group. ZBPYR improved the learning and memory impairment, which might be related to the maintenance of dendritic spine density in different brain regions.
