Study on Improvement of Chemosensitivity of MCF-7 Cells to Epirubicin and Inhibition of Aurora Kinase A in Treatment of Breast Cancer by San-Huang Decoction
10.11842/wst.2015.10.019
- VernacularTitle:三黄煎剂抑制Aurora激酶A增强表柔比星对乳腺癌MCF-7细胞药效的研究＊
- Author:
Yanlei XU
;
Xu CHEN
;
Xiyan CHEN
;
Weihe BIAN
;
Chang YAO
;
Xiaoshu ZHU
;
Xiaozhou YE
- Publication Type:Journal Article
- Keywords:
San-Huang Decoction;
Aurora Kinase A;
MCF-7 cells;
cell proliferation;
apoptosis
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(10):2060-2068
- CountryChina
- Language:Chinese
-
Abstract:
This article was aimed to explore the effect ofSan-Huang (SH) decoction on improving chemosensitivity of MCF-7 cells to epirubicin and inhibition of Aurora kinase A, in order to discuss its underlying mechanism. The inhibition of MCF-7 cells proliferation on breast cancer by SH decoction was determined by CCK-8 assay. RT-PCR and western blot were used to detect the Aurora A, p53 mRNA and protein expression level of MCF-7 cells by SH decoction. The siRNA silenced Aurora A of MCF-7 cells. CCK-8 assay was used to detect the inhibition of MCF-7 cells proliferation. CCK-8 assay and AnnexinV-FITC/PI staining were used to detect the inhibition rate and apoptosis rate of MCF-7 cells treated by the combination of SH decoction and epirubicin. Western blot analysis was used to detect the expression of apoptosis-related proteins. The results showed that SH decoction inhibited the proliferation of MCF-7 cells in a dose-dependent manner (P< 0.05). The effect of 48 h medication was better than 24 h (P < 0.05). There was no statistical difference with medication for 72 h (P > 0.05). SH decoction can regulate the Aurora A, p53 protein and mRNA expression of MCF-7 cells. siRNA silenced Aurora A, which downregulated the inhibition rate of MCF-7 cells by SH decoction for 50.0% (from 49.2% to 24.8%). The combination of SH decoction and epirubicin enhanced the effect of epirubicin on inhibiting the proliferation rate and apoptosis rate of MCF-7 cell, regulated the expression levels of apoptosis-related protein such as c-PARP, c-Caspase 3, Bcl-2, Bax, as well as the protein level of Aurora A. It was concluded that SH decoction can increase the chemosensitivity of MCF-7 cells to epirubicin, which may be related to the inhibition of Aurora Kinase A by SH decoction.
