Effect ofXiao-Qing-LongDecoction on AngⅡ, ALD, Na+-k+-ATPase Contents and Expressions of AT1 and AT2 mRNA in Cor pulmonale Rats
10.11842/wst.2015.08.027
- VernacularTitle:小青龙汤对肺源性心脏病大鼠血浆AngⅡ、ALD、Na+-K+-ATP酶含量和血浆中AT1和AT2mRNA的表达影响*
- Author:
Jiabing TONG
;
Hao WANG
;
Zegeng LI
;
Chuanbo WANG
;
Cheng YANG
- Publication Type:Journal Article
- Keywords:
Xiao-Qing-Long Decoction;
ventilating lung qi for dieresis;
AngⅡ;
fluorescence quantitative
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(10):2104-2108
- CountryChina
- Language:Chinese
-
Abstract:
This article was aimed to study the effect ofXiao-Qing-LongDecoction (XQLD) on plasma AngⅡ, ALD, Na+-k+-ATPase content, and plasma AT1 and AT2 mRNA expressions in Cor pulmonale rats, in order to further explore the mechanism of ventilating lung qi for diuresis. A total of 60 Wistar rats were randomly divided into the normal group, model group and XQLD, with 20 rats in each group. The enzyme-linked immunosorbent assay (ELISA) method was used to determine the AngⅡ, ALD and Na+-k+-ATPase content. The fluorescence quantitative PCR was used to detect the AT1 and AT2 mRNA expression. The results showed that compared with the normal group, the AngⅡ, ALD and Na+-k+-ATPase contents in the model group were significantly increased (P< 0.05, orP< 0.01). Compared with the model group, the AngⅡ, ALD and Na+-k+-ATPase contents in the XQLD group were obviously decreased (P< 0.05). Compared with the normal group, AT1 mRNA expression was increased; and AT2 mRNA expression was decreased in the model group (P<0.05, orP< 0.01). Compared with the model group, AT1 mRNA expression was decreased; and AT2 mRNA expression was increased in the XQLD group (P < 0.01). It was concluded that XQLD can effectively regulate the AT1 and AT2 mRNA expressions, influence ALD content to ventilate lungqi for dieresis.