UV radiation-induced DNA damage in keratinocytes and the protective effect of resveratrol
10.3969/j.issn.1006-5725.2015.23.003
- VernacularTitle:紫外线对皮肤角质形成细胞DNA的损伤效应以及白藜芦醇的保护作用
- Author:
Hongxia LIU
;
Liang ZHOU
;
Zhenhua DING
- Publication Type:Journal Article
- Keywords:
Ultraviolet;
HaCaT;
DNA damage;
Comet assay;
Resveratrol
- From:
The Journal of Practical Medicine
2015;31(23):3822-3825
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of ultraviolet on DNA damage in keratinocytes and to observe the protective role of resveratrol for the cells. Methods Comet assay was employed to evaluate the damage after radiation with different doses of UV rays (UVA, UVB and UVC) of 0, 10, 30, 50, 70 and 90 mJ/cm2, and the effects after pretreatment with various concentrations of resveratrol under irradiation with 30 mJ/cm2. Results UVA irradiation (0 ~ 90 mJ/cm2) had no significant effects on HaCaT cells. However, TailDNA%, TailLength, CometLength, TailMoment and OliveTailMoment showed both UVB and UVC induced DNA damage in a dose-de-pentent manner. UVC was more harmful than UVB at the same dose. Conclusions The DNA breakage induced by UVB and UVC is dose-dependent. As compared with UVB, UVC is more harmful to HaCaT cells. Resveratrol exerts a protective effect in HaCaT cells irradiated by UVB or UVC.
