Research on Proteomic Spectra Expression of Traditional Chinese Medicine Cold and Heat Constitution Rats Based on Two-dimensional Gel Electrophoresis
10.11842/wst.2015.10.016
- VernacularTitle:基于双向凝胶电泳技术的中医寒、热体大鼠蛋白质组表达谱研究＊
- Author:
Weifei ZHANG
;
Fufeng LI
;
Zhumei SUN
;
Ruiyi ZHANG
;
Chunrong GUO
;
Weirong ZHANG
- Publication Type:Journal Article
- Keywords:
Cold constitution;
heat constitution;
differential protein;
two-dimensional gel electrophoresis;
mass spectrometry
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(10):2044-2048
- CountryChina
- Language:Chinese
-
Abstract:
This article was aimed to study the proteomic spectra expression of traditional Chinese medicine (TCM) cold and heat constitution rats with two-dimensional gel electrophoresis (2-DE), in order to search for the cold and heat-associated proteins for the investigation of the biological basis of TCM cold and heat body constitution formation. The total protein in rat’s liver cell was extracted. The 2-DE and MALDI-TOF/TOF mass spectrometry (MS) were used in the screening and identification of differentially expressed proteins of cold and heat constitution rats. The results showed that a total of 10 different points in the protein expression were obtained with statistical significance after screening and MS, which were carbamoyl-phosphate synthase, protein disulfide isomerase associated 3, catalase, hydroperoxide isomerase, cytosol aminopeptidase, glutamate dehydrogenase 1, 3-hydroxy-3-methylglutaryl-coenzyme A synthase 2, heat shock protein 60 (Hsp60) precursor, homocysteine, 78 kDa glucose-regulated protein precursor. It was concluded that some differences were existed in the proteomic spectra expression of TCM cold and heat constitution rats. The abnormality of enzyme protein metabolism may be one of the material bases for the formation of cold and heat constitution.
