Correlation between CYP2J2 gene polymorphism and coronary heart disease in Chinese Han population and effects of CYP2J2 geneover-expressionon aortic smooth muscle cells of ApoE-/-mice
10.3969/j.issn.1006-5725.2015.23.005
- VernacularTitle:CYP2J2基因多态性与中国汉族人冠心病的关系及其过表达对ApoE-/-小鼠主动脉平滑肌细胞的影响
- Author:
Rui LI
;
Jian QIU
;
Lei SHI
;
Yuan ZHANG
;
Huacheng YAN
;
Yunjun RUAN
;
Hua XIAO
- Publication Type:Journal Article
- Keywords:
Coronary disease;
CYP2J2;
Genepolymorphism;
Smooth muscle cells;
Cell prolifera-tion;
Cell migration
- From:
The Journal of Practical Medicine
2015;31(23):3831-3836
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between CYP2J2*7 mutation(G-76T) and coronary heart disease (CHD) in Chinese Hanpopulation and to study the effects of CYP2J2 geneover-expressionon the proliferation and migrationof aortic smooth muscle cells of ApoE-/- mice. Methods CYP2J2*7 genotype was detectedin 500 patients with CHD and 478 controlsubjects by the Polymerase Chain Reaction-Restriction Frag-ment Length Polymorphism (PCR-RFLP). Culturedaortic smooth muscle cells of ApoE-/- mice were divided into control group, sham transfectiongroup and CYP2J2 over-expression group. Cell proliferation and migration were investigated after CYP2J2 over-expressionby MTS and Transwell assay. Results The frequency of CYP2J2*7 in CHD group was significantly higher than that incontrol group (10.00% vs. 6.49%, P = 0.046). Same is the case in female cases(P = 0.026). Compared with these of aortic smooth muscle cells incontrol group and sham trans-fectiongroup, the cell proliferation in 24, 48, 72 h, and the cell migration in 48 h after CYP2J2 over-expression in CYP2J2 group were significantly suppressed. Conclusions CYP2J2*7 mutation might increase the risk of CHD in Chinese Han population. CYP2J2 over-expression can suppress the proliferation and migration of aortic smooth muscle cells and CYP2J2 might have the effect of anti-atherosclerosis.
