Detection of TORCH infection in pregnant women by using reverse phase protein array
10.3969/j.issn.1673-4130.2015.24.006
- VernacularTitle:反向蛋白质微点阵对怀孕女性 TORC H感染的检测
- Author:
Wenjun HE
;
Fang TANG
;
Tao LI
;
Zian WU
;
Xinzhong WU
;
Fan JIANG
;
Liandong ZUO
;
Tingyu YU
;
Zhirong TAN
;
Ning XU
- Publication Type:Journal Article
- Keywords:
reverse phase protein array;
TORCH infection;
enzyme linked immunosorbent assay
- From:
International Journal of Laboratory Medicine
2015;(24):3522-3524
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate whether the reverse phase protein array (RPPA) method can be used for detecting TORCH infection in pregnant women .Methods The RPPA method was established for detecting TORCH infection .The positive coinci‐dence rates of TORCH infection detected by the RPPA method and ELISA method in 2000 fresh serum samples from pregnant women were compared for evaluating the feasibility of RPPA in TORCH detection .Results The positive coincidence rates of estab‐lished RPPA and ELISA for detecting TORCH infection was 100 .0% ,91 .1% ,97 .2% ,91 .3% and 93 .0% respectively ,indicating that the detection results of various indexes by RPPA and ELISA had better consistency (P>0 .05) ,but the positive detection rates of RPPA for Rubellavirus ,CMV and HSV‐1 ,2 were higher than those of correspondent ELISA method .Conclusion RPPA method for detecting TORCH infection has the advantages of simpleness ,rapidness ,high sensitivity and strong specificity ,is an effective method of auxiliary diagnosis for bearing and rearing better children in clinical ,and is worthy of being promoted and used in the fu‐ture .
